Typhiwell

Typhiwell


An Enzyme – Linked Immunosorbent Assay (ELISA) for the
detection of IgM antibodies specific to Salmonella typhi
in human serum/plasma

FEATURES

  • Able to run 96 samples at a time
  • Break apart wells
  • Detecting IgM for acute typhoid fever
  • Total assay time 75 Minutes
  • Unmasking IgM results in equivocal diagnosis of typhoid fever

PRODUCT DESCRIPTION

  • Method : detection of IgM antibodies to the extracted proteins of Salmonella typhi
  • Assay type : Enzyme Linked Immunosorbent Assay (ELISA)
  • Sample : Human serum or plasma
  • Sample volume : 2 μl
  • Storage : 2-8 º C
  • Sensitivity : 96.8 %
  • Specificity : 92.1%

PRINCIPLE OF THE TEST

The microwells are coated with specific typhoid antigen. Diluted patients serum, after removal of human IgG interference and rheumatoid factor, is added to the microwell. IgM specific antibody, if present,binds to the antigen. All unbound materials are washed away during washing step before anti human hrp conjugated is added to the antigen – antibody complexes, if present. The whole complex were then visualised by adding a chromogen (Tetramethylbenzidine or TMB). Addition of Stop Solution ends the reaction and turns the blue colour to bright yellow colour. The reaction will then be read by an ELISA reader.

INTRODUCTION

Typhoid fever is endemic in Asia-Pacific region, the Indian Sub continental, Africa and South America. WHO has estimated 22
million new cases worldwide with about 200,000 deaths. Typhoid is an infectious disease caused by bacteria called Salmonella
typhi. The sources of infectious agent are human carriers and it is transmitted by faecal-oral route when food or water
contaminated with infected faeces is ingested.
In endemic area, due to reinfection, the secondary immune response will boost the IgG concentration compared to IgM, thus
results in false negative IgM results.

TEST INTERPRETATION

  • Samples interpreted as non-reactive (0-0.49) indicate antibody is not present in the sample
  • Samples interpreted as reactive (> 0.5) indicate the presence of specific antibody towards Salmonella typhi
tyhpiwell_new